Time-resolved transient absorption ultrasonic microscopy measurements of the ground state recovery time

Ryan Shelton; Scott Mattison; Brian E. Applegate

doi:10.1117/12.909415

23 February 2012 Time-resolved transient absorption ultrasonic microscopy measurements of the ground state recovery time

Ryan Shelton, Scott Mattison, Brian E. Applegate

Author Affiliations +

Proceedings Volume 8223, Photons Plus Ultrasound: Imaging and Sensing 2012; 82233U (2012) https://doi.org/10.1117/12.909415
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We have recently developed Transient Absorption Ultrasonic Microscopy (TAUM) as an ultrahigh-resolution photoacoustic microscopy technique. The amplitude of the multiphoton pump-probe interaction is dependent on the interpulse delay between the pump and probe pulses. Measuring the interpulse delay dependent TAUM amplitude maps out the ground state recovery time of the chromophore. The ground state recovery time is a molecular signature that may be used to differentiate multiple chromophores, analogous to fluorescence lifetime. We have used TAUM to measure the ground state recovery time of Rhodamine 6G to be 3.65 ns, which matches well with known literature values. Whole blood is also investigated, with measured ground state recovery times of 3.74 ns for oxygenated blood and 7.9 ns for deoxygenated blood. The distinct difference in lifetimes for the oxidized and reduced forms suggests the feasibility of subcellular SO2 images maps in future iterations of TAUM.

Citation Download Citation

Ryan Shelton, Scott Mattison, and Brian E. Applegate "Time-resolved transient absorption ultrasonic microscopy measurements of the ground state recovery time", Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 82233U (23 February 2012); https://doi.org/10.1117/12.909415

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KEYWORDS

Blood

Chromophores

Absorption

Luminescence

Microscopy

Ultrasonics

Photoacoustic spectroscopy

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