Multispectral angular domain imaging with a tunable pulsed laser

Eldon Ng; Fartash Vasefi; Jeffrey J. L. Carson

doi:10.1117/12.909763

9 February 2012 Multispectral angular domain imaging with a tunable pulsed laser

Eldon Ng, Fartash Vasefi, Jeffrey J. L. Carson

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82252C (2012) https://doi.org/10.1117/12.909763
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Angular Domain Imaging (ADI) is an imaging technique that is capable of generating three dimensional images of attenuating targets embedded in a scattering medium. In ADI, an angular filter is positioned between the sample and the detector to discriminate between quasi-ballistic photons and scattered photons. Quasi-ballistic photons have undergone relatively few forward directed scattering events, and can be used to generate a projection image representative of the imaging target. Scattered photons have undergone many scattering events and contain little information regarding the imaging target, thereby leading to decreased image contrast. Our implementation of ADI utilizes a silicon micro-channel array to reject scattered photons based on the angle at which they exit the sample. The objective of this work was to collect ADI images with a tunable pulsed laser within the visible range. Samples were illuminated at 13 wavelengths between 460 nm and 700 nm. An angular filter array of 80 μm × 80 μm tunnels 2-cm long was used to select the quasiballistic photons. Images were detected with a linear 16-bit linear CCD. The phantom consisted of a 0.7 mm attenuating target submerged in one of four Intralipid^R dilutions (0.15%-0.3%) contained within a 1 cm path length cuvette. Image contrast ranged from 0.02 at 460 nm and 0.3% Intralipid^R to 1 at 680 nm at 0.15% Intralipid^R. For a given scattering level, contrast increased at longer wavelengths. Resolution varied minimally with wavelength. The results suggested that multispectral ADI with a tunable pulsed laser is feasible and may find utility in imaging thin tissue samples in the future.

Citation Download Citation

Eldon Ng, Fartash Vasefi, and Jeffrey J. L. Carson "Multispectral angular domain imaging with a tunable pulsed laser", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252C (9 February 2012); https://doi.org/10.1117/12.909763

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available