Tissue imaging with a stigmatic mass microscope using laser desorption/ionization

Kunio Awazu; Hisanao Hazama; Tomonori Hamanaka; Jun Aoki; Michisato Toyoda; Yasuhide Naito

doi:10.1117/12.909898

9 February 2012 Tissue imaging with a stigmatic mass microscope using laser desorption/ionization

Kunio Awazu, Hisanao Hazama, Tomonori Hamanaka, Jun Aoki, Michisato Toyoda, Yasuhide Naito

Proceedings Volume 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X; 82252E (2012) https://doi.org/10.1117/12.909898
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

A novel stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight mass spectrometer, MULTUM-IMG, has been developed. Stigmatic ion images of crystal violet masked by a fine square mesh grid with a 12.7 μm pitch were clearly observed, and the estimated spatial resolution was about 3 μm in the linear mode with a 20-fold ion optical magnification. Tissue sections of a brain and eyes of a mouse stained with crystal violet and methylene blue were observed in the linear mode, and the stigmatic total ion images of crystal violet and methylene blue agreed well with the optical photomicrograph of the same sections. Especially, the fine structure in the cornea tissue was clearly observed with a spatial resolution in the range of micrometers. Although the total measurement time of the stigmatic ion image for the whole-eye section was about 59 minutes using a laser with a 10 Hz repetition rate, the measurement time could be reduced to about 35 s using a laser with a 1 kHz repetition rate and automation of measurements. The stigmatic mass microscope developed in this research should be suitable for high-spatial resolution and high-throughput imaging mass spectrometry for pathology, pharmacokinetics, and so on.

Citation Download Citation

Kunio Awazu, Hisanao Hazama, Tomonori Hamanaka, Jun Aoki, Michisato Toyoda, and Yasuhide Naito "Tissue imaging with a stigmatic mass microscope using laser desorption/ionization", Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252E (9 February 2012); https://doi.org/10.1117/12.909898

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KEYWORDS

Ions

Microscopes

Spatial resolution

Cornea

Tissue optics

Electrodes

Photomicroscopy

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