Anti-VEGF therapies have been widely explored for the management of posterior ocular disease, like neovascular
age-related macular degeneration (AMD). Loading anti-VEGF therapies in biodegradable microparticles may enable
sustained drug release and improved therapeutic outcome. However, existing microfabrication processes such as double
emulsification produce drug-loaded microparticles with low encapsulation rate and poor antibody bioactivity. To
overcome these limitations, we fabricate multifunctional microparticles by both single needle and coaxial needle
electrospray. The experimental setup for the process includes flat-end syringe needles (both single needle and coaxial
needle), high voltage power supplies, and syringe pumps. Microparticles are formed by an electrical field between the
needles and the ground electrode. Droplet size and morphology are controlled by multiple process parameters and
material properties, such as flow rate and applied voltage. The droplets are collected and freezing dried to obtain
multifunctional microparticles. Fluorescent beads encapsulated poly(DL-lactide-co-glycolide) acid (PLGA)
microparticles are injected into rabbits eyes through intravitreal injection to test the biodegradable time of microparticles.
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