The development of highly specific markers for fluorescent microscopy has become a very popular research topic.
Organic fluorophores have several drawbacks, such as photobleaching and autofluorescence. Therefore increasing
interest in inorganic nanoparticles has been observed because of their unseen photostability, chemical robustness and
straightforward synthesis. The surface of iron oxide nanoparticles was coated with trialkoxy silanes, which introduced
functional groups for possible subsequent coupling reactions. An additional gold layer was added to the surface of the
particle to show the enhanced contrast improvement. The nanoparticles were imaged by an optical microscope, in dark
field mode, on a glass substrate and inside microorganisms. This proved that the reported method could have great
potential as a labelling technique, since it combines the non-photobleaching, photostable nanoparticles with a
straightforward and rapid imaging technique.
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