Enhancing antibiofilm efficacy in antimicrobial photodynamic therapy: effect of microbubbles

Anil Kishen; Saji George

doi:10.1117/12.2005908

1 March 2013 Enhancing antibiofilm efficacy in antimicrobial photodynamic therapy: effect of microbubbles

Anil Kishen, Saji George

Proceedings Volume 8580, Dynamics and Fluctuations in Biomedical Photonics X; 85800B (2013) https://doi.org/10.1117/12.2005908
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this study, we tested the hypothesis that a microbubble containing photosensitizer when activated with light would enable comprehensive disinfection of bacterial biofilms in infected root dentin by antimicrobial photodynamic therapy (APDT). Experiments were conducted in two stages. In the stage-1, microbubble containing photosensitizing formulation was tested for its photochemical properties. In the stage-2, the efficacy of microbubble containing photosensitizing formulation was tested on in vitro infected root canal model, developed with monospecies biofilm models of Enterococcus faecalis on root dentin substrate. The findings from this study showed that the microbubble containing photosensitizing formulation was overall the most effective formulation for photooxidation, generation of singlet oxygen, and in disinfecting the biofilm bacteria in the infected root canal model. This modified photosensitizing formulation will have potential advantages in eliminating bacterial biofilms from infected root dentin.

Citation Download Citation

Anil Kishen and Saji George "Enhancing antibiofilm efficacy in antimicrobial photodynamic therapy: effect of microbubbles", Proc. SPIE 8580, Dynamics and Fluctuations in Biomedical Photonics X, 85800B (1 March 2013); https://doi.org/10.1117/12.2005908

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available