Single shot white light interference microscopy with colour fringe analysis for quantitative phase imaging of biological cells

Vishal Srivastava; D. S. Mehta

doi:10.1117/12.2003994

22 February 2013 Single shot white light interference microscopy with colour fringe analysis for quantitative phase imaging of biological cells

Vishal Srivastava, D. S. Mehta

Author Affiliations +

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 858706 (2013) https://doi.org/10.1117/12.2003994
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

To quantitatively obtain the phase map of Onion and human red blood cell (RBC) from white light interferogram we used Hilbert transform color fringe analysis technique. The three Red, Blue and Green color components are decomposed from single white light interferogram and Refractive index profile for Red, Blue and Green colour were computed in a completely non-invasive manner for Onion and human RBC. The present technique might be useful for non-invasive determination of the refractive index variation within cells and tissues and morphological features of sample with ease of operation and low cost.

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Vishal Srivastava and D. S. Mehta "Single shot white light interference microscopy with colour fringe analysis for quantitative phase imaging of biological cells", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 858706 (22 February 2013); https://doi.org/10.1117/12.2003994

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