In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy

Yuji Tanaka; Eiji Hase; Shuichiro Fukushima; Takeshi Yasui; Tsutomu Araki

doi:10.1117/12.2003969

22 February 2013 In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy

Yuji Tanaka, Eiji Hase, Shuichiro Fukushima, Takeshi Yasui, Tsutomu Araki

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85882X (2013) https://doi.org/10.1117/12.2003969
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Polarization-resolved second-harmonic-generation (SHG) microscopy is a powerful tool to visualize distribution of collagen fiber orientation in tissue with little invasion. However, long image acquisition time, resulting from mechanical rotation of a half-wave plate, makes this microscopy easy to suffer from motion artifact of a sample and hence has limited its use to in vivo application. In this paper, we constructed rapid, polarization-resolved SHG microscopy by combination of an electro-optics-modulator-based polarization modulation with improved data acquisition method. The constructed polarization-resolved SHG microscopy enables us to visualize orientation mapping of dermal collagen fiber in rat skin and human one in vivo without influence of motion artifact. This microscope will open the door for in vivo measurement of collagen fiber orientation in human skin.

Citation Download Citation

Yuji Tanaka, Eiji Hase, Shuichiro Fukushima, Takeshi Yasui, and Tsutomu Araki "In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882X (22 February 2013); https://doi.org/10.1117/12.2003969

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