Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge; Yixin Wang; Cuifang Kuang; Shin-Shian Lee; Nirmal Mazumder; Fu-Jen Kao

doi:10.1117/12.2009387

22 February 2013 Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin-Shian Lee, Nirmal Mazumder, Fu-Jen Kao

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858832 (2013) https://doi.org/10.1117/12.2009387
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Citation Download Citation

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin-Shian Lee, Nirmal Mazumder, and Fu-Jen Kao "Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858832 (22 February 2013); https://doi.org/10.1117/12.2009387

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available