Detecting apoptosis in vivo and ex vivo using spectroscopic OCT and dynamic light scattering

Golnaz Farhat; Anoja Giles; Adrian Mariampillai; Victor X. D. Yang; Gregory J. Czarnota; Michael C. Kolios

doi:10.1117/12.2040075

11 March 2014 Detecting apoptosis in vivo and ex vivo using spectroscopic OCT and dynamic light scattering

Golnaz Farhat, Anoja Giles, Adrian Mariampillai, Victor X. D. Yang, Gregory J. Czarnota, Michael C. Kolios

Proceedings Volume 8952, Biomedical Applications of Light Scattering VIII; 89520K (2014) https://doi.org/10.1117/12.2040075
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

We present an in vivo implementation of a multi-parametric technique for detecting apoptosis using optical coherence tomography in a mouse tumor model. Solid tumors were grown from acute myeloid leukemia cells in the hind leg of SCID mice and treated with a single dose of cisplatin and dexamethasone to induce apoptosis. Both spectral features and speckle decorrelation times indicated good consistency between control mice and reasonable agreement with in vitro measurements. The integrated backscatter increased significantly in tumors responding to treatment while the spectral slope and decorrelation time did not show significant changes. This study demonstrates the feasibility of using spectroscopic OCT and dynamic light scattering for treatment monitoring in vivo.

Citation Download Citation

Golnaz Farhat, Anoja Giles, Adrian Mariampillai, Victor X. D. Yang, Gregory J. Czarnota, and Michael C. Kolios "Detecting apoptosis in vivo and ex vivo using spectroscopic OCT and dynamic light scattering", Proc. SPIE 8952, Biomedical Applications of Light Scattering VIII, 89520K (11 March 2014); https://doi.org/10.1117/12.2040075

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