In-Vivo Fluorescence Spectroscopy Of Normal And Atherosclerotic Arteries

Lawrence I. Deckelbaum; Ian J. Sarembock; Mark L. Stetz; Kenneth M. O'Brien; Francis W. Cutruzzola; Arthur F. Gmitro; Michael D. Ezekowitz

doi:10.1117/12.945298

21 June 1988 In-Vivo Fluorescence Spectroscopy Of Normal And Atherosclerotic Arteries

Lawrence I. Deckelbaum, Ian J. Sarembock, Mark L. Stetz, Kenneth M. O'Brien, Francis W. Cutruzzola, Arthur F. Gmitro, Michael D. Ezekowitz

Author Affiliations +

Proceedings Volume 0906, Optical Fibers in Medicine III; (1988) https://doi.org/10.1117/12.945298
Event: 1988 Los Angeles Symposium: O-E/LASE '88, 1988, Los Angeles, CA, United States

Abstract

Laser-induced fluorescence spectroscopy can discriminate atherosclerotic from normal arteries in-vitro and may thus potentially guide laser angioplasty. To evaluate the feasibility of laser-induced fluorescence spectroscopy in a living blood-filled arterial system we performed fiberoptic laser-induced fluorescence spectroscopy in a rabbit model of focal femoral atherosclerosis. A laser-induced fluorescence spectroscopy score was derived from stepwise linear regression analysis of in-vitro spectra to distinguish normal aorta (score>0) from atherosclerotic femoral artery (score<0). A 400 u silica fiber, coupled to a helium cadmium laser and optical multichannel analyzer, was inserted through a 5F catheter to induce and record in-vivo fluorescence from femoral and aortoiliac arteries. Arterial spectra could be recorded in all animals (n=10: 5 occlusions, 5 stenoses). Blood spectra were of low intensity and were easily distinguished from arterial spectra. The scores (mean ± SEM) for the in-vivo spectra were -0.69 ± 0.29 for artherosclerotic femoral, and +0.54 ±. 0.15 for normal aorta (p<.01; p=NS compared to in-vitro spectra). In-vitro, a fiber tip to tissue distance <50 u was necessary for adequate arterial LIFS in blood. At larger distances low intensity blood spectra were recorded (1/20 the intensity of tissue spectra). Thus, fiberoptic laser-induced fluorescence spectroscopy can be sucessfully performed in a blood filled artery provided the fiber tip is approximated to the tissue.

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Lawrence I. Deckelbaum, Ian J. Sarembock, Mark L. Stetz, Kenneth M. O'Brien, Francis W. Cutruzzola, Arthur F. Gmitro, and Michael D. Ezekowitz "In-Vivo Fluorescence Spectroscopy Of Normal And Atherosclerotic Arteries", Proc. SPIE 0906, Optical Fibers in Medicine III, (21 June 1988); https://doi.org/10.1117/12.945298

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