In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy

Zhibin Wang; Lin Wei; Yi He; Xiqi Li; Guohua Shi; Yudong Zhang

doi:10.1117/12.2068823

17 September 2014 In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy

Zhibin Wang, Lin Wei, Yi He, Xiqi Li, Guohua Shi, Yudong Zhang

Proceedings Volume 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014); 92301W (2014) https://doi.org/10.1117/12.2068823
Event: Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 2014, Wuhan, China

Abstract

Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures.

Citation Download Citation

Zhibin Wang, Lin Wei, Yi He, Xiqi Li, Guohua Shi, and Yudong Zhang "In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy", Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301W (17 September 2014); https://doi.org/10.1117/12.2068823

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