Evaluation of laser photobiomodulation on bone defect in the femur of osteoporotic rats: a Raman spectral study

Luiz Guilherme P. Soares; Jouber Mateus dos Santos Aciole; Bruno Luiz R. C. Neves; Landulfo Silveira Jr.; Antônio L. B. Pinheiro D.D.S.

doi:10.1117/12.2079248

5 March 2015 Evaluation of laser photobiomodulation on bone defect in the femur of osteoporotic rats: a Raman spectral study

Luiz Guilherme P. Soares, Jouber Mateus dos Santos Aciole, Bruno Luiz R. C. Neves, Landulfo Silveira Jr., Antônio L. B. Pinheiro D.D.S.

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Proceedings Volume 9309, Mechanisms for Low-Light Therapy X; 930910 (2015) https://doi.org/10.1117/12.2079248
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Phototherapies have shown positive effects on the bone repair process, increasing the blood supply to the injured area. The aim of this study was to assess through Raman spectroscopy, the efficacy of laser phototherapy (λ = 780 nm, P = 70 mW, CW, 20.4 J/cm² per session, 163.2 J/cm² per treatment) on the bone repair process of osteoporotic rats. The osteoporosis induction was achieved by ovariectomy surgery. Thirty Wistar rats were divided into 4 groups (Basal; OVX, OVX + Clot and OVX + Clot + Laser), then subdivided into 2 subgroups according to the experimental time (15 and 30 days). After the osteoporosis induction time (60 days), a bone defect with 2 mm was created with a trephine drill in the right femur in the animals of groups OVX, Clot and Clot + Laser. After surgery, the irradiation protocol was applied in the same groups on repeated sessions every 48 hours during 15 days. The samples were analyzed by Raman Spectroscopy to assess the inorganic content of phosphate and carbonated hydroxyapatite (~960 and 1070 cm^-1, respectively) and organic lipids and proteins (~1454 cm^-1). Statistical analysis (ANOVA, Student-T test) showed significant difference between groups Basal, OVX + Clot, and OVX + Clot + Laser for the inorganic content peaks at ~960 (p≤0.001), and ~1070 cm^-1 (p≤0.001) in both periods of 15 and 30 days, however on peak at ~1450 cm⁻¹ no differences were detected. It was concluded that the Laser phototherapy increased deposition of HA on bone repair process of osteoporotic rats.

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Luiz Guilherme P. Soares, Jouber Mateus dos Santos Aciole, Bruno Luiz R. C. Neves, Landulfo Silveira Jr., and Antônio L. B. Pinheiro D.D.S. "Evaluation of laser photobiomodulation on bone defect in the femur of osteoporotic rats: a Raman spectral study", Proc. SPIE 9309, Mechanisms for Low-Light Therapy X, 930910 (5 March 2015); https://doi.org/10.1117/12.2079248

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KEYWORDS

Bone

Raman spectroscopy

Laser dentistry

Statistical analysis

Phototherapy

Surgery

Proteins

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