Non-invasive discrimination between pancreatic islets and exocrine cells using multiphoton microscopy

Binlin Wu; Ge Li; Mingming Hao; Sushmita Mukherjee

doi:10.1117/12.2083555

27 April 2015 Non-invasive discrimination between pancreatic islets and exocrine cells using multiphoton microscopy

Binlin Wu, Ge Li, Mingming Hao, Sushmita Mukherjee

Proceedings Volume 9329, Multiphoton Microscopy in the Biomedical Sciences XV; 932935 (2015) https://doi.org/10.1117/12.2083555
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

In this study, we propose a non-invasive method to distinguish pancreatic islet cells from exocrine cell clusters using multiphoton (MP) imaging. We demonstrate the principle of distinguishing them based on autofluorescence. The results show that MP imaging has a potential to distinguish pancreatic islets from exocrine cells. This ability to distinguish the two cell types could have many applications, such as the examination of fresh pancreatic biopsies when staining is not possible or desirable.

Citation Download Citation

Binlin Wu, Ge Li, Mingming Hao, and Sushmita Mukherjee "Non-invasive discrimination between pancreatic islets and exocrine cells using multiphoton microscopy", Proc. SPIE 9329, Multiphoton Microscopy in the Biomedical Sciences XV, 932935 (27 April 2015); https://doi.org/10.1117/12.2083555

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