Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

Thorsten Vahlsing; Sven Delbeck; Janpeter Budde; Lars Cocchieri; Dieter Ihrig; Steffen Leonhardt; H. Michael Heise

doi:10.1117/12.2080289

9 March 2015 Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Lars Cocchieri, Dieter Ihrig, Steffen Leonhardt, H. Michael Heise

Author Affiliations +

Proceedings Volume 9332, Optical Diagnostics and Sensing XV: Toward Point-of-Care Diagnostics; 933209 (2015) https://doi.org/10.1117/12.2080289
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Micro-dialysis has been established in the clinical environment for continuously harvesting body fluids, but a drawback of this process are variable recovery rates, which can be observed especially for subcutaneously implanted catheters. Perfusates with either acetate or mannitol have been investigated as recovery markers. The latter substance is suggested for application with external cavity tuneable quantum cascade lasers, rendering a limited wavenumber interval in contrast to FTIR-spectrometers. Despite the overlap of mannitol and glucose spectra, their simultaneous quantification was successful. By investigating the depletion of the marker substances from the perfusates using different micro-dialysis devices, the theoretical nonlinear relationship between the relative dialysate marker concentration and glucose recovery rate was confirmed for the marker substance-analyte pair of acetate and glucose, rendering a basis for reliable blood glucose measurements. For the pair of mannitol and glucose an almost linear dependency was expected for the microdialysate catheters and experimentally verified, which provides a straightforward correction of any dialysis recovery rate variation during patient monitoring.

Citation Download Citation

Thorsten Vahlsing, Sven Delbeck, Janpeter Budde, Lars Cocchieri, Dieter Ihrig, Steffen Leonhardt, and H. Michael Heise "Ex-vivo glucose sensors using micro-dialysis: importance of on-line recovery rate determination by multi-analyte infrared spectrometry", Proc. SPIE 9332, Optical Diagnostics and Sensing XV: Toward Point-of-Care Diagnostics, 933209 (9 March 2015); https://doi.org/10.1117/12.2080289

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available