Differentiating neutrophils using the optical coulter counter

E. Schonbrun; G. Di Caprio

doi:10.1117/12.2079141

11 March 2015 Differentiating neutrophils using the optical coulter counter

E. Schonbrun, G. Di Caprio

Proceedings Volume 9336, Quantitative Phase Imaging; 933614 (2015) https://doi.org/10.1117/12.2079141
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

We present an opto-fluidic measurement system that quantifies cell volume, dry mass and nuclear morphology of neutrophils in high-throughput. While current clinical hematology analyzers can differentiate neutrophils from a blood sample, they do not give other quantitative information beyond their count. In order to better understand the distribution of neutrophil phenotypes in a blood sample, we perform two distinct multivariate measurements. In both measurements, white blood cells are driven through a microfluidic channel and imaged while in flow onto a color camera using a single exposure. In the first measurement, we quantify cell volume, scattering strength, and cell dry mass by combining quantitative phase imaging with dye exclusion cell volumetric imaging. In the second measurement, we quantify cell volume and nuclear morphology using a nucleic acid fluorescent stain. In this way, we can correlate cell volume to other cellular characteristics, which would not be possible using an electrical coulter counter. Unlike phase imaging or cell scattering analysis, the optical coulter counter is capable of quantifying cell volume virtually independent of the cell’s refractive index and unlike optical tomography, measurements are possible on quickly flowing cells, enabling high-throughput.

Citation Download Citation

E. Schonbrun and G. Di Caprio "Differentiating neutrophils using the optical coulter counter", Proc. SPIE 9336, Quantitative Phase Imaging, 933614 (11 March 2015); https://doi.org/10.1117/12.2079141

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KEYWORDS

Blood

Scattering

Phase imaging

Refractive index

Cameras

Microfluidics

Image transmission

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