Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival

José D. Vollet Filho; Marina R. da Silveira; Orlando Castro-e-Silva; Vanderlei S. Bagnato; Cristina Kurachi

doi:10.1117/12.2180730

19 June 2015 Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival

José D. Vollet Filho, Marina R. da Silveira, Orlando Castro-e-Silva, Vanderlei S. Bagnato, Cristina Kurachi

Author Affiliations +

Proceedings Volume 9531, Biophotonics South America; 95310X (2015) https://doi.org/10.1117/12.2180730
Event: SPIE Biophotonics South America, 2015, Rio de Janeiro, Brazil

Abstract

Evaluating transplantation grafts at harvest is essential for its success. Laser-induced fluorescence spectroscopy (LIFS) can help monitoring changes in metabolic/structural conditions of tissue during transplantation. The aim of the present study is to correlate LIFSobtained spectra of human hepatic grafts during liver transplantation with post-operative patients' mortality rate and biochemical parameters, establishing a method to exclude nonviable grafts before implantation. Orthotopic liver transplantation, piggyback technique was performed in 15 patients. LIFS was performed under 408nm excitation. Collection was performed immediately after opening donor’s abdominal cavity, after cold perfusion, end of back-table period, and 5 min and 1 h after warm perfusion at recipient. Fluorescence information was compared to lactate, creatinine, bilirubin and INR levels and to survival status. LIFS was sensitive to liver changes during transplantation stages. Study-in-progress; initial results indicate correlation between fluorescence and life/death status of patients.

Citation Download Citation

José D. Vollet Filho, Marina R. da Silveira, Orlando Castro-e-Silva, Vanderlei S. Bagnato, and Cristina Kurachi "Fluorescence spectroscopy for assessment of liver transplantation grafts concerning graft viability and patient survival", Proc. SPIE 9531, Biophotonics South America, 95310X (19 June 2015); https://doi.org/10.1117/12.2180730

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