Ex-vivo assessment of tissue viability using dynamic laser speckle

E. E Ramírez-Miquet; L. M. Miquet Romero; J. G. Darias; R. A. Martínez-Celorio

doi:10.1117/12.2196321

24 August 2015 Ex-vivo assessment of tissue viability using dynamic laser speckle

E. E Ramírez-Miquet, L. M. Miquet Romero, J. G. Darias, R. A. Martínez-Celorio

Proceedings Volume 9660, SPECKLE 2015: VI International Conference on Speckle Metrology; 96601U (2015) https://doi.org/10.1117/12.2196321
Event: SPECKLE 2015: VI International Conference on Speckle Metrology, 2015, Guanajuato, Mexico

Abstract

Dynamic laser speckle is a non-destructive contactless sensing method useful for exploring activity inherent to biological samples. We present an ex-vivo analysis of dermal and epidermal tissue with different degrees of activity in healthy and burned tissue. Pseudocolor images obtained after processing biospeckle stacks with the generalized differences reveal a correlation between cellular lysis and speckle pattern activity. Epidermis shows higher activity than dermal tissue, which is attributable to the number of cells and each tissue. The analysis presented here could be employed in assessment of viability of tissues for graft and burns treatments.

Citation Download Citation

E. E Ramírez-Miquet, L. M. Miquet Romero, J. G. Darias, and R. A. Martínez-Celorio "Ex-vivo assessment of tissue viability using dynamic laser speckle", Proc. SPIE 9660, SPECKLE 2015: VI International Conference on Speckle Metrology, 96601U (24 August 2015); https://doi.org/10.1117/12.2196321

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