Effects of short term changes in the blood glucose level on the autofluorescence lifetime of the human retina in healthy volunteers

Matthias Klemm; Edgar Nagel; Dietrich Schweitzer; Stefan Schramm; Jens Haueisen

doi:10.1117/12.2208605

4 March 2016 Effects of short term changes in the blood glucose level on the autofluorescence lifetime of the human retina in healthy volunteers

Matthias Klemm, Edgar Nagel, Dietrich Schweitzer, Stefan Schramm, Jens Haueisen

Author Affiliations +

Proceedings Volume 9693, Ophthalmic Technologies XXVI; 96931R (2016) https://doi.org/10.1117/12.2208605
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Purpose: Fluorescence lifetime imaging ophthalmoscopy (FLIO) provides in vivo metabolic mapping of the ocular fundus. Changes in FLIO have been found in e.g. diabetes patients. The influence of short term metabolic changes caused by blood glucose level changes on is unknown. Aim of this work is the detection of short-term changes in fundus autofluorescence lifetime during an oral glucose tolerance test. Methods: FLIO was performed in 10 healthy volunteers (29±4 years, fasting for 12h) using a scanning laser ophthalmoscope (30° fundus, 34μm resolution, excitation with 473nm diode laser with 70 ps pulses at 80 MHz repetition rate, detection in two spectral channels 500-560nm (ch1) and 560-720nm (ch2) using the timecorrelated single photon counting method). The blood glucose level (BGL) was measured by an Accu-Chek® Aviva self-monitoring device. Before and after a glucose drink (300ml solution, containing 75g of glucose (Accu-Chek® Dextrose O.G.T.), BGL and FLIO were measured every 15min. The FLIMX software package was applied to compute the average fluorescence lifetime τ on the inner ring of the ETDRS grid using a modified 3-exponential approach. Results: The results are given as mean ± standard deviation over all volunteers in ch1. Baseline measurement: BGL: 5.3±0.4 mmol/l, τ₁: 49±6ps. A significant reduction (α=5%; Wilcoxon rank-sum test) in τ₁ is detected after 15min (BGL: 8.4±1.1 mmol/l, τ₁: 44±5ps) and after 90min (BGL: 6.3±1.4 mmol/l, τ₁: 41±5ps). Results of ch2 show smaller reductions in the fluorescence lifetimes over time.

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Matthias Klemm, Edgar Nagel, Dietrich Schweitzer, Stefan Schramm, and Jens Haueisen "Effects of short term changes in the blood glucose level on the autofluorescence lifetime of the human retina in healthy volunteers", Proc. SPIE 9693, Ophthalmic Technologies XXVI, 96931R (4 March 2016); https://doi.org/10.1117/12.2208605

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