Evaluation of PpIX formation in Cervical Intraepithelial Neoplasia I (CIN) using widefield fluorescence images

Fernanda M. Carbinatto; Natalia Mayumi Inada; Thereza C. Fortunato; Welington Lombardi; Eduardo Venerando da Silva; José Dirceu Vollet Filho; Cristina Kurachi; Sebastião Pratavieira; Vanderlei Salvador Bagnato

doi:10.1117/12.2213250

7 March 2016 Evaluation of PpIX formation in Cervical Intraepithelial Neoplasia I (CIN) using widefield fluorescence images

Fernanda M. Carbinatto, Natalia Mayumi Inada, Thereza C. Fortunato, Welington Lombardi, Eduardo Venerando da Silva, José Dirceu Vollet Filho, Cristina Kurachi, Sebastião Pratavieira, Vanderlei Salvador Bagnato

Author Affiliations +

Proceedings Volume 9699, Optics and Biophotonics in Low-Resource Settings II; 96990Z (2016) https://doi.org/10.1117/12.2213250
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Optical techniques has been described as auxiliary technology for screening of neoplasia because shows the potential for tissues differentiation in real-time and it is a noninvasive detection and safe. However, only endogenous fluorophores presents the lesion may be insufficient and needed of the administration of the fluorophores synthesized, such as, precursor molecule of protoporphyrin IX (PpIX) induced by 5- aminolevulinic acid and your derivatives. Topical application of methylaminolevulinate (MAL), induces formation of the endogenous photosensitizer, PpIX in tissues where carcinogenesis has begun. The PpIX tend to accumulate in premalignant and malignant tissues and the illumination with light with appropriate wavelength beginning to excitation of PpIX fluorescence, which helps to localize PpIX-rich areas and identify potentially malignant tissues. The aim of the study is to evaluate the production of PpIX in the cervix with CIN I through of the fluorescence images captured after 1 hour of cream application. It was possible to visualize PpIX fluorescence in cervix and it was possible to observe the selectivity in fluorescence in squamous-columnar junction, which a pre-cancerous condition (CIN) and usually is localized. Through the image processing it was possible to quantify the increase of red fluorescence. For the CIN I the increase of red fluorescence was approximately of 4 times indicating a good PpIX formation.

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Fernanda M. Carbinatto, Natalia Mayumi Inada, Thereza C. Fortunato, Welington Lombardi, Eduardo Venerando da Silva, José Dirceu Vollet Filho, Cristina Kurachi, Sebastião Pratavieira, and Vanderlei Salvador Bagnato "Evaluation of PpIX formation in Cervical Intraepithelial Neoplasia I (CIN) using widefield fluorescence images", Proc. SPIE 9699, Optics and Biophotonics in Low-Resource Settings II, 96990Z (7 March 2016); https://doi.org/10.1117/12.2213250

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