All-in-one detector of circulating mRNA based on a smartphone

Vratislav Cmiel; Jaromir Gumulec; Ondrej Svoboda; Martina Raudenska; Kristyna Hudcova; Jiri Sekora; Jaroslav Balogh; Michal Masarik; Ivo Provaznik

doi:10.1117/12.2213253

4 March 2016 All-in-one detector of circulating mRNA based on a smartphone

Vratislav Cmiel, Jaromir Gumulec, Ondrej Svoboda, Martina Raudenska, Kristyna Hudcova, Jiri Sekora, Jaroslav Balogh, Michal Masarik, Ivo Provaznik

Author Affiliations +

Proceedings Volume 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics; 971509 (2016) https://doi.org/10.1117/12.2213253
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

Citation Download Citation

Vratislav Cmiel, Jaromir Gumulec, Ondrej Svoboda, Martina Raudenska, Kristyna Hudcova, Jiri Sekora, Jaroslav Balogh, Michal Masarik, and Ivo Provaznik "All-in-one detector of circulating mRNA based on a smartphone", Proc. SPIE 9715, Optical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics, 971509 (4 March 2016); https://doi.org/10.1117/12.2213253

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available