Paper
9 March 2016 Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media
M. Lostak, J. Collakova, T. Slaby, A. Krizova, P. Vesely, R. Chmelik
Author Affiliations +
Proceedings Volume 9718, Quantitative Phase Imaging II; 97181Y (2016) https://doi.org/10.1117/12.2209322
Event: SPIE BiOS, 2016, San Francisco, California, United States
Abstract
In this work we present the coherence controlled holographic microscopy (CCHM)1 and its ability to image the living cells in turbid media2.

The CCHM method and its advantages are introduced. A 'coherence gate effect'3, that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.
© (2016) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
M. Lostak, J. Collakova, T. Slaby, A. Krizova, P. Vesely, and R. Chmelik "Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media", Proc. SPIE 9718, Quantitative Phase Imaging II, 97181Y (9 March 2016); https://doi.org/10.1117/12.2209322
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Coherence (optics)

Holography

Diffusers

Microscopy

Phase imaging

Autoregressive models

Light

Back to Top