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28 April 2022 Rapid random z-access two-photon light-sheet microscope for neural imaging
Dongli Xu, Jun Ding, Leilei Peng
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Proceedings Volume PC11946, Neural Imaging and Sensing 2022; PC119460O (2022) https://doi.org/10.1117/12.2610667
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Light-sheet fluorescence microscopy serves as a fast high-resolution imaging method for neural imaging. However, its 3D imaging ability is often limited by the speed of scanning the detection focal plane in the z-direction. Herein, we develop a rapid random z-access two-photon light-sheet microscope, which incorporates a two-photon Bessel beam light-sheet microscope with a dynamically driven electrical tunable lens (ETL). With a precise ETL calibration process and a novel rapid random z-access method, our system can selectively scan any desired z-section in the 3D imaging volume at the speed of 100 frame-per-second, and allows neural activates monitoring on the living brain tissue at video rate.
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Dongli Xu, Jun Ding, and Leilei Peng "Rapid random z-access two-photon light-sheet microscope for neural imaging", Proc. SPIE PC11946, Neural Imaging and Sensing 2022, PC119460O (28 April 2022); https://doi.org/10.1117/12.2610667
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KEYWORDS
Microscopes
Tissue optics
Stereoscopy
Calibration
Fluorescence spectroscopy
Luminescence
Microscopy
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