In-vivo fluorescence lifetime tomography for detection and quantification of immunotherapy marker, PD-L1

Rahul Pal; Aya Matsui; Murali K.; Satoru Morita; Homan Kang; Hak Soo Choi; Dan G. Duda; Anand Kumar

doi:10.1117/12.2650664

7 March 2023 In-vivo fluorescence lifetime tomography for detection and quantification of immunotherapy marker, PD-L1

Rahul Pal, Aya Matsui, Murali K., Satoru Morita, Homan Kang, Hak Soo Choi, Dan G. Duda, Anand Kumar

Proceedings Volume PC12376, Optical Tomography and Spectroscopy of Tissue XV; PC123760P (2023) https://doi.org/10.1117/12.2650664
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Programed death ligand-1 (PD-L1) expression is currently the only predictive biomarker for cancer immunotherapy. Since PD-L1 expression in tumors is largely heterogeneous, in-vivo detection and quantification of PD-L1 in intact tumors is of major interest. Here we employ fluorescence lifetime (FLT) imaging for in-vivo detection and quantification of PD-L1 expression using an anti-PD-L1 antibody conjugated to IRDye800CW (αPDL1-800). We show that FLT imaging accurately identifies heterogeneous PD-L1 expression in tumors. Tumor areas of high PD-L1 levels were spatially correlated to significantly longer FLTs of αPDL1-800 and the distribution of PD-L1 in deep-seated (>1cm depth) tumors was achieved using FLT tomography.

Conference Presentation

Citation Download Citation

Rahul Pal, Aya Matsui, Murali K., Satoru Morita, Homan Kang, Hak Soo Choi, Dan G. Duda, and Anand Kumar "In-vivo fluorescence lifetime tomography for detection and quantification of immunotherapy marker, PD-L1", Proc. SPIE PC12376, Optical Tomography and Spectroscopy of Tissue XV, PC123760P (7 March 2023); https://doi.org/10.1117/12.2650664

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available