Acoustically induced sample reorientation for multi-angle OCT

Monika Ritsch-Marte; Wolfgang Drexler; Mia Kvåle Løvmo; Simon Moser; Shiyu Deng; Rainer Leitgeb

doi:10.1117/12.3002921

13 March 2024 Acoustically induced sample reorientation for multi-angle OCT

Monika Ritsch-Marte, Wolfgang Drexler, Mia Kvåle Løvmo, Simon Moser, Shiyu Deng, Rainer Leitgeb

Proceedings Volume PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; PC1284803 (2024) https://doi.org/10.1117/12.3002921
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Optical Coherence Tomography (OCT) is a valuable tool for label-free imaging with micrometer resolution. However, conventional approaches to OCT typically image a sample from a single direction or angle, which limits its ability to image structures placed behind strongly attenuating material or deep inside the object. Here we introduce a solution to this problem by adding a small chamber to a spectral-domain OCT setup. Acoustic actuation enables a contact-free levitation and stepwise reorientation of samples such as zebra-fish larvae and tumor spheroids in a controlled and reproducible manner. We further developed a model-based compressive algorithm, which is able to exploit the diverse multi-angle OCT volumes for a 3D-reconstruction with isotropic resolution and estimation of refractive index values. We demonstrate and validate our approach on zebrafish larvae. We believe that our approach represents a powerful enabling tool for developmental biology and for organoid and cancer spheroid research.

Conference Presentation

Citation Download Citation

Monika Ritsch-Marte, Wolfgang Drexler, Mia Kvåle Løvmo, Simon Moser, Shiyu Deng, and Rainer Leitgeb "Acoustically induced sample reorientation for multi-angle OCT", Proc. SPIE PC12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, PC1284803 (13 March 2024); https://doi.org/10.1117/12.3002921

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