Presentation
7 March 2022 Applying sparse spectral sampling SRS microscopy to evaluate topical pharmacokinetics
Author Affiliations +
Abstract
Stimulated Raman scattering (SRS) is a rapid, nondestructive technique for imaging biomedical specimens with label-free chemical specificity. Here, we demonstrate a sparse spectral sampling SRS imaging approach where a rapidly-tunable dual-output all-fiber optical parametric oscillator is automatically tuned into specific vibrational modes across >1400cm-1. We demonstrate this system via simultaneously monitoring an active pharmaceutical ingredient and excipient within a single formulation, tracking penetration and permeation through distinct layers of ex vivo mouse skin over time with Raman fingerprint and high wavenumber spectral contrast. This novel method is ideally suited to monitor spatiotemporal dynamic changes during topical drug delivery.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Isaac J. Pence, Benjamin A. Kuzma, and Conor L. Evans "Applying sparse spectral sampling SRS microscopy to evaluate topical pharmacokinetics", Proc. SPIE PC11938, Visualizing and Quantifying Drug Distribution in Tissue VI, PC119380E (7 March 2022); https://doi.org/10.1117/12.2608810
Advertisement
Advertisement
KEYWORDS
Microscopy

Raman spectroscopy

Imaging spectroscopy

Light sources

Nondestructive evaluation

Optical parametric oscillators

Raman scattering

Back to Top