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2 March 2022 psiSCAT and RF-iSCAT: nanometric techniques to access less explored dimensions of nano-scale objects
Il-Buem Lee, Hyun-Min Moon, Jin-Sung Park, Seok-Cheol Hong, Minhaeng Cho
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Proceedings Volume PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022; PC119720M (2022) https://doi.org/10.1117/12.2610454
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Being a label-free technique, iSCAT has emerged as a powerful tool complementary to fluorescence microscopy because its superb spatio-temporal resolution holds great promise in biological investigations ranging from detection of nm-sized proteins to real-time dynamics of organelles in vivo. Here we present the polarization-selective iSCAT (psiSCAT) microscopy to detect the orientation and anisotropy of nano-objects. We also succeeded in developing the remote-focusing iSCAT (RF-iSCAT) to realize fast, vibration-free, 3D imaging over a long z-tracking range (< 5 μm). We envisage that our achievement would greatly advance the field of single-particle tracking into new dimensions with superb precision and sensitivity.
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Il-Buem Lee, Hyun-Min Moon, Jin-Sung Park, Seok-Cheol Hong, and Minhaeng Cho "psiSCAT and RF-iSCAT: nanometric techniques to access less explored dimensions of nano-scale objects", Proc. SPIE PC11972, Label-free Biomedical Imaging and Sensing (LBIS) 2022, PC119720M (2 March 2022); https://doi.org/10.1117/12.2610454
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KEYWORDS
Microscopy
3D image processing
Anisotropy
In vivo imaging
Interferometry
Optical microscopy
Proteins
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