Optofluidic technology for monitoring rotifer Brachionus calyciflorus responses to regular light pulses

Rhys Cartlidge; Olivia Campana; Dayanthi Nugegoda; Donald Wlodkowic

doi:10.1117/12.2242879

9 December 2016 Optofluidic technology for monitoring rotifer Brachionus calyciflorus responses to regular light pulses

Rhys Cartlidge, Olivia Campana, Dayanthi Nugegoda, Donald Wlodkowic

Author Affiliations +

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100132B (2016) https://doi.org/10.1117/12.2242879
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

Behavioural alterations can occur as a result of a toxicant exposure at concentrations significantly lower than lethal effects that are commonly measured in acute toxicity testing. The use of alternating light and dark photoperiods to test phototactic responses of aquatic invertebrates in the presence of environmental contaminants provides an attractive analytical avenue. Quantification of phototactic responses represents a sublethal endpoint that can be employed as an early warning signal. Despite the benefits associated with the assessment of these endpoints, there is currently a lack of automated and miniaturized bioanalytical technologies to implement the development of toxicity testing with small aquatic species. In this study we present a proof-of-concept microfluidic Lab-on-a-Chip (LOC) platform for the assessment of rotifer swimming behavior in the presence of the toxicant copper sulfate. The device was designed to assess impact of toxicants at sub-lethal concentrations on freshwater crustacean Brachionus calyciflorus, testing behavioral endpoints such as animal swimming distance, speed and acceleration. The LOC device presented in this work enabled straightforward caging of microscopic crustaceans as well as non-invasive analysis of rapidly swimming animals in a focal plane of a video-microscopy system. The chip-based technology was fabricated using a new photolithography method that enabled formation of thick photoresist layers with minimal distortion. Photoresist molds were then employed for replica molding of LOC devices with poly(dimethylsiloxane) (PDMS) elastomer. The complete bioanalytical system consisted of: (i) microfluidic PDMS chip-based device; (ii) peristaltic microperfusion pumping manifold; (iii) miniaturized CMOS camera for video data acquisition; and (iv) video analysis software algorithms for quantification of changes in swimming behaviour of B. calyciflorus in response to reference toxicants.

Citation Download Citation

Rhys Cartlidge, Olivia Campana, Dayanthi Nugegoda, and Donald Wlodkowic "Optofluidic technology for monitoring rotifer Brachionus calyciflorus responses to regular light pulses", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100132B (9 December 2016); https://doi.org/10.1117/12.2242879

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