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Stimulated Raman scattering (SRS) microscopy is a promising technique for label-free imaging of living systems. We demonstrate microsecond-scale SRS spectral imaging by tuning two spectrally focused pulses temporally through a resonant delay-line. Our platform acquired an SRS spectrum within 42 microseconds and formed a spectral image composed of 40,000 pixels in real-time.
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Chien-Sheng Liao, Kai-Chih Huang, Weili Hong, Andy J. Chen, Caroline Karanja, Pu Wang, Gregory Eakins, Ji-Xin Cheng, "Stimulated Raman spectroscopic imaging by microsecond delay-line tuning," Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 1006911 (21 February 2017); https://doi.org/10.1117/12.2256802