CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10074 > Article
Open Access Presentation
24 April 2017 Halo-free phase contrast microscopy (Conference Presentation)
Tan H. Nguyen, Mikhail E. Kandel, Haadi M. Shakir, Catherine Best, Minh N. Do, Gabriel Popescu
Author Affiliations +
Proceedings Volume 10074, Quantitative Phase Imaging III; 1007404 (2017) https://doi.org/10.1117/12.2256892
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
The phase contrast (PC) method is one of the most impactful developments in the four-century long history of microscopy. It allows for intrinsic, nondestructive contrast of transparent specimens, such as live cells. However, PC is plagued by the halo artifact, a result of insufficient spatial coherence in the illumination field, which limits its applicability. We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Measuring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission function by using a physical model of the image formation under partial spatial coherence. Using this halo-free sample transmission, we can numerically generate artifact-free PC images. Furthermore, this transmission can be further used to obtain quantitative information about the sample, e.g., the thickness with known refractive indices, dry mass of live cells during their cycles. We tested our hfPC method on various control samples, e.g., beads, pillars and validated its potential for biological investigation by imaging live HeLa cells, red blood cells, and neurons.
Conference Presentation

View presentation recording on the SPIE Digital Library: http://dx.doi.org/10.1117/12.2256892.5380600139001
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Tan H. Nguyen, Mikhail E. Kandel, Haadi M. Shakir, Catherine Best, Minh N. Do, and Gabriel Popescu "Halo-free phase contrast microscopy (Conference Presentation)", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007404 (24 April 2017); https://doi.org/10.1117/12.2256892
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Microscopy
Phase contrast
Phase measurement
Spatial coherence
Image acquisition
Interferometry
Microscopes
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top