Characterization of microsieves recovery efficiency in isolation of circulating tumor cells

Paulina Natalia Osuchowska; Antoni Sarzyński; Marek Strzelec; Zdzisław Bogdanowicz; Jan Marczak; Mariusz Piotr Łapiński; Elżbieta Anna Trafny

doi:10.1117/12.2262369

8 December 2016 Characterization of microsieves recovery efficiency in isolation of circulating tumor cells

Paulina Natalia Osuchowska, Antoni Sarzyński, Marek Strzelec, Zdzisław Bogdanowicz, Jan Marczak, Mariusz Piotr Łapiński, Elżbieta Anna Trafny

Author Affiliations +

Proceedings Volume 10159, Laser Technology 2016: Progress and Applications of Lasers; 101590D (2016) https://doi.org/10.1117/12.2262369
Event: XIth Symposium on Laser Technology, 2016, Jastarnia, Poland

Abstract

Isolation of circulating tumor cells (CTCs) from the blood is important in the diagnosis of malignant tumors and for monitoring therapeutic responses. The two main problems to be solved are extremely low CTCs numbers in the blood (average 1-10 CTC per 10 ml of whole blood) and the absence of one particular phenotype or genotype, which would allow for precise identification. Isolation of CTCs can be based on physical characteristics, e.g. the size of the cells (ISET, Isolation by Size of Epithelial Tumor cells) or the biological properties of these cells (the expression of specific proteins on their surface). In the IOE WAT the copper alloy microsieves with a pore diameter of 10.85 ± 0.89 μm designed for cell isolation by ISET method were produced. The microsieves with 100 000 pores with a 50 μm interval was made using precise, percussion laser drilling. The performance microsieves filtration was determined using fluorescent beads with three dimensions: 4 μm, 10 μm and 15 μm. Furthermore, the suspensions of cells lines from different types of tumor were used in the process of filtration. The efficiency of the cells filtration process was affected by lack of biocompatibility of the material used for the microsieves production as well as the roughness and porosity of the microsieves surface. Moreover, the diameter of the pores and the course of the filtration process were also significant.

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Paulina Natalia Osuchowska, Antoni Sarzyński, Marek Strzelec, Zdzisław Bogdanowicz, Jan Marczak, Mariusz Piotr Łapiński, and Elżbieta Anna Trafny "Characterization of microsieves recovery efficiency in isolation of circulating tumor cells ", Proc. SPIE 10159, Laser Technology 2016: Progress and Applications of Lasers, 101590D (8 December 2016); https://doi.org/10.1117/12.2262369

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