Multiphoton fluorescence spectroscopy optical fiber sensor

Maciej Popenda; Hanna Stawska; Elżbieta Bereś-Pawlik

doi:10.1117/12.2271071

10 February 2017 Multiphoton fluorescence spectroscopy optical fiber sensor

Maciej Popenda, Hanna Stawska, Elżbieta Bereś-Pawlik

Proceedings Volume 10325, Optical Fibers and Their Applications 2017; 103250H (2017) https://doi.org/10.1117/12.2271071
Event: 17th Conference on Optical Fibres and Their Applications, 2017, Supraśl, Poland, Poland

Abstract

In this paper, we present the project and usage of multiphoton fluorescence spectroscopy sensor, based on the photonic crystal fiber as an excitation pulse transmission medium and four POF fibers acting as an emission collection elements. Usage of the photonic crystal fiber as a transmission medium allowed us to transmit the 780 nm excitation pulse with total time spread as low as 65 fs, which resulted in pulse broadening from the base 127 fs to 192 fs. Pulse was focused onto the sample via the GRIN lens, which resulted in an observable fluorescence. Collection tips of the POF fibers were additionally angled to move the collection cones towards the GRIN lens focal point, which resulted in further increase of the collection efficiency. This allowed to create a sensor capable of measuring fluorescence emission of the fluorescein solution with concentration as low as 10^-4 M, with very low amount of bulk optics between the sensor and the spectrometer.

Citation Download Citation

Maciej Popenda, Hanna Stawska, and Elżbieta Bereś-Pawlik "Multiphoton fluorescence spectroscopy optical fiber sensor", Proc. SPIE 10325, Optical Fibers and Their Applications 2017, 103250H (10 February 2017); https://doi.org/10.1117/12.2271071

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