Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

Philip Wijesinghe; Niloufer J. Johansen; Andrea Curatolo; David D. Sampson; Ruth Ganss; Brendan F. Kennedy

doi:10.1117/12.2269903

29 April 2017 Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta

Philip Wijesinghe, Niloufer J. Johansen, Andrea Curatolo, David D. Sampson, Ruth Ganss, Brendan F. Kennedy

Proceedings Volume 10340, International Conference on Biophotonics V; 1034010 (2017) https://doi.org/10.1117/12.2269903
Event: International Conference on Biophotonics V, 2017, Perth, Australia

Abstract

We have developed a high-resolution optical coherence elastography system capable of estimating Young’s modulus in tissue volumes with an isotropic resolution of 15 μm over a 1 mm lateral field of view and a 100 μm axial depth of field. We demonstrate our technique on healthy and hypertensive, freshly excised and intact mouse aortas. Our technique has the capacity to delineate the individual mechanics of elastic lamellae and vascular smooth muscle. Further, we observe global and regional vascular stiffening in hypertensive aortas, and note the presence of local micro-mechanical signatures, characteristic of fibrous and lipid-rich regions.

Citation Download Citation

Philip Wijesinghe, Niloufer J. Johansen, Andrea Curatolo, David D. Sampson, Ruth Ganss, and Brendan F. Kennedy "Optical coherence elastography for cellular-scale stiffness imaging of mouse aorta", Proc. SPIE 10340, International Conference on Biophotonics V, 1034010 (29 April 2017); https://doi.org/10.1117/12.2269903

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