Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution

Arash Darafsheh

doi:10.1117/12.2289240

23 February 2018 Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution

Arash Darafsheh

Author Affiliations +

Proceedings Volume 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV; 104990W (2018) https://doi.org/10.1117/12.2289240
Event: SPIE BiOS, 2018, San Francisco, California, United States

Abstract

Microsphere-assisted imaging can be incorporated onto conventional light microscopes allowing wide-field and flourescence imaging with enhanced resolution. We demonstrated that imaging of specimens containing subdiffraction-limited features is achievable through high-index microspheres embedded in a transparent thin film placed over the specimen. We fabricated novel microsphere-embedded microscope slides composed of barium titanate glass microspheres (with diameter ~10-100 μm and refractive index~1.9-2.2) embedded in a transparent polydimethylsiloxane (PDMS) elastomer layer with controllable thickness. We characterized the imaging performance of such microsphere-embedded devices in white-light microscopies, by measuring the imaging resolution, field-of-view, and magnification as a function of microsphere size. Our results inform on the design of novel optical devices, such as microsphere-embedded microscope slides for imaging applications.

Citation Download Citation

Arash Darafsheh "Fabrication and characterization of novel microsphere-embedded optical devices for enhancing microscopy resolution", Proc. SPIE 10499, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXV, 104990W (23 February 2018); https://doi.org/10.1117/12.2289240

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