Differential expression of myofibroblasts on CO2 laser wounds and scalpel wounds: an experimental model

R. M. Machado; C. R. B. Oliveira; L. A. Vitória; F. C. A. Xavier; A. L. B. Pinheiro; A. C. Freitas; L. M. P. Ramalho

doi:10.1117/12.2315599

12 April 2018 Differential expression of myofibroblasts on CO₂ laser wounds and scalpel wounds: an experimental model

R. M. Machado, C. R. B. Oliveira, L. A. Vitória, F. C. A. Xavier, A. L. B. Pinheiro, A. C. Freitas, L. M. P. Ramalho

Proceedings Volume 10582, Laser Florence 2017: Advances in Laser Medicine; 105820I (2018) https://doi.org/10.1117/12.2315599
Event: Laser Florence 2017, 2017, Florence, Italy

Abstract

Wound contraction of both traumatic and surgical origin may reduce or limit the function of the tissue. Myofibroblasts are cells involved on the process of wound contraction, which is smaller on CO₂ Laser wounds. The aims of this study were to quantitative and statistically assess the presence of myofibroblasts on both conventional and CO₂ Laser wounds. Thirty-two animals (rattus norvegicus) were divided into four groups and operated using either the CO₂ Laser (groups A1 and A2) or conventional scalpel (groups B1 and B2). The animals were sacrificed eight days post-operatively (groups: A1 and B1) and 14^th days after surgery (groups: A2 and B2). The spec imens we re routinely processed to wax and stained with a-Smooth Muscle Actin (aSMA) and analyzed under light microscopy (40X). Two standard areas around the wound of each slide were selected and used to count the number of myofribroblasts present using a calibrated eyepiece and a graticule. The number of myofibroblasts at day eight was significantly higher than at day 14^th. Comparison of the two techniques at day eight showed significant differences between the two groups (Laser, p=0.007 and scalpel, p=0.001). The number of cells present on group B1 was significantly higher than group A1 (p=0.001). However at the 14^th day there was no such difference (p=0,072). It is concluded that the small number of myofibroblasts at day eight after wounding with the CO₂ Lasermay be the reason why contraction on this wound is smaller than the one observed in conventional surgery.

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R. M. Machado, C. R. B. Oliveira, L. A. Vitória, F. C. A. Xavier, A. L. B. Pinheiro, A. C. Freitas, and L. M. P. Ramalho "Differential expression of myofibroblasts on CO₂ laser wounds and scalpel wounds: an experimental model", Proc. SPIE 10582, Laser Florence 2017: Advances in Laser Medicine, 105820I (12 April 2018); https://doi.org/10.1117/12.2315599

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KEYWORDS

Gas lasers

Carbon dioxide lasers

Laser dentistry

Laser therapeutics

Wound healing

Carbon monoxide

Tissues

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