Assessing embryo development using swept source optical coherence tomography

S. Caujolle; R. Cernat; G. Silvestri; M. J. Marques; A. Bradu; T. Feuchter; G. Robinson; D. Griffin; A. Podoleanu

doi:10.1117/12.2282912

5 March 2018 Assessing embryo development using swept source optical coherence tomography

S. Caujolle, R. Cernat, G. Silvestri, M. J. Marques, A. Bradu, T. Feuchter, G. Robinson, D. Griffin, A. Podoleanu

Proceedings Volume 10591, 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources; 1059108 (2018) https://doi.org/10.1117/12.2282912
Event: Second Canterbury Conference on Optical Coherence Tomography, 2017, Canterbury, United Kingdom

Abstract

A detailed assessment of embryo development would assist biologists with selecting the most suitable embryos for transfer leading to higher pregnancy rates. Currently, only low resolution microscopy is employed to perform this assessment. Although this method delivers some information on the embryo surface morphology, no specific details are shown related to its inner structure. Using a Master-Slave Swept-Source Optical Coherence Tomography (SS-OCT), images of bovine embryos from day 7 after fertilization were collected from different depths. The dynamic changes inside the embryos were examined, in detail and in real-time from several depths. To prove our ability to characterize the morphology, a single embryo was imaged over 26 hours. The embryo was deprived of its life support environment, leading to its death. Over this period, clear morphological changes were observed.

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S. Caujolle, R. Cernat, G. Silvestri, M. J. Marques, A. Bradu, T. Feuchter, G. Robinson, D. Griffin, and A. Podoleanu "Assessing embryo development using swept source optical coherence tomography", Proc. SPIE 10591, 2nd Canterbury Conference on OCT with Emphasis on Broadband Optical Sources, 1059108 (5 March 2018); https://doi.org/10.1117/12.2282912

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