Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes

Yi-Hung Liao; Chia-Lung Hsieh

doi:10.1117/12.2319565

24 April 2018 Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes

Yi-Hung Liao, Chia-Lung Hsieh

Author Affiliations +

Proceedings Volume 10711, Biomedical Imaging and Sensing Conference; 1071105 (2018) https://doi.org/10.1117/12.2319565
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

Following the journey of a single molecule is a powerful tool for studying the structures and functions of biological membranes. To resolve the interplays between individual membrane molecules and their nano-associations, membrane dynamics needs to be measured at the nanoscale with high temporal resolution. Here, we demonstrate ultrahigh-speed coherent brightfield (COBRI) microscopy for capturing diffusive motion of single lipid molecules in model membranes labeled by single 40 nm gold nanoparticles with a spatial precision of 3.6 nm and a temporal resolution of 2.56 microseconds (corresponding to a frame rate of 390 kHz) under an illumination intensity of 15 μW/μm2. Our method is readily applicable to the future study of plasma membrane of live cells where the transient confinements in membrane nanodomains could be investigated with unprecedented clarity.

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Yi-Hung Liao and Chia-Lung Hsieh "Coherent brightfield (COBRI) microscopy for ultrahigh-speed single-particle tracking on lipid bilayer membranes", Proc. SPIE 10711, Biomedical Imaging and Sensing Conference, 1071105 (24 April 2018); https://doi.org/10.1117/12.2319565

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KEYWORDS

Microscopy

Molecules

Temporal resolution

Diffusion

Laser scattering

Light scattering

Visualization

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