Multi-dimensional digital holographic microscopy

Osamu Matoba; Xiangyu Quan; Manoj Kumar; Yasuhiro Awatsuji

doi:10.1117/12.2501202

5 November 2018 Multi-dimensional digital holographic microscopy

Osamu Matoba, Xiangyu Quan, Manoj Kumar, Yasuhiro Awatsuji

Author Affiliations +

Proceedings Volume 10815, Optical Design and Testing VIII; 108150T (2018) https://doi.org/10.1117/12.2501202
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

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Osamu Matoba, Xiangyu Quan, Manoj Kumar, and Yasuhiro Awatsuji "Multi-dimensional digital holographic microscopy ", Proc. SPIE 10815, Optical Design and Testing VIII, 108150T (5 November 2018); https://doi.org/10.1117/12.2501202

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