High-resolution molecular spectroscopy and biological applications

Sarah K. Scholten; Joshua Whitaker-Lockwood; Christopher Perrella; Faisal Karim; Andre N. Luiten

doi:10.1117/12.2541241

30 December 2019 High-resolution molecular spectroscopy and biological applications

Sarah K. Scholten, Joshua Whitaker-Lockwood, Christopher Perrella, Faisal Karim, Andre N. Luiten

Proceedings Volume 11200, AOS Australian Conference on Optical Fibre Technology (ACOFT) and Australian Conference on Optics, Lasers, and Spectroscopy (ACOLS) 2019; 112001Y (2019) https://doi.org/10.1117/12.2541241
Event: ANZCOP, 2019, Melbourne, Australia

Abstract

The rapid measurement of gas concentrations is beneficial to a broad range of applications, including pollution monitoring, high-precision spectroscopy, and medical breath analysis. Optical frequency combs (OFCs) are a near-ideal optical interrogation source for molecular spectroscopy, combining broadband light with high frequency-precision and dense spectral sampling, characteristics which also allow short measurement times. A spectrometer based on a virtually imaged phased array (VIPA) is used to unravel the comb into a high-resolution transmission spectrum, with the returned spectra rapidly fitted with a detailed spectral model for concentration extraction of CO₂. We demonstrate an accuracy of 0.5% and 12% for concentration measurements of ¹²C¹⁶O₂ and ¹³C¹⁶O₂ respectively, with the measured isotopic ratio in excellent agreement with that expected from their natural abundances. Precision of the concentration measurements is also high, at 0.03% and 1.24% for ¹²C¹⁶O₂ and ¹³C¹⁶O₂ respectively. The measurement technique is also verified to be highly linear for concentrations ranging over three orders of magnitude. The spatial and temporal coherence of OFCs additionally enables the use of the full range of performance-enhancing optical techniques including resonant enhancement. Here we demonstrate the use of a multi-pass Herriott cell to enhance the effective measurement path length, which allows the continuous optical measurement of CO₂ production during the respiration of baker’s yeast in a closed system. Results from the yeast experiments agree well with existing literature.

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Sarah K. Scholten, Joshua Whitaker-Lockwood, Christopher Perrella, Faisal Karim, and Andre N. Luiten "High-resolution molecular spectroscopy and biological applications", Proc. SPIE 11200, AOS Australian Conference on Optical Fibre Technology (ACOFT) and Australian Conference on Optics, Lasers, and Spectroscopy (ACOLS) 2019, 112001Y (30 December 2019); https://doi.org/10.1117/12.2541241

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KEYWORDS

Molecular spectroscopy

Spectroscopy

Phased array optics

Frequency combs

Yeast

Phased arrays

Oxygen

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