Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells

Yanping He; Yijin Wang; Renjie Zhou

doi:10.1117/12.2547017

28 February 2020 Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells

Yanping He, Yijin Wang, Renjie Zhou

Author Affiliations +

Proceedings Volume 11294, Emerging Digital Micromirror Device Based Systems and Applications XII; 1129402 (2020) https://doi.org/10.1117/12.2547017
Event: SPIE OPTO, 2020, San Francisco, California, United States

Abstract

The development of high throughput three-dimensional (3D) microscopic imaging technique is important for studying cell physiology and early-stage disease diagnoses. Here we propose and demonstrate a digital micromirror device (DMD) based angle-multiplexed high-speed optical diffraction tomography (ODT) technique. Using this ODT technique, we have achieved 3D imaging of cells at over 600 tomogram/second speed, which is 10-100 times faster than current ODTbased 3D cell imaging techniques. We envision that this high-speed ODT system will enable many cutting-edge biomedical applications, such as capturing millisecond scale cell dynamics in 3D space and high throughput 3D imaging of large cell populations.

Citation Download Citation

Yanping He, Yijin Wang, and Renjie Zhou "Digital micromirror device based angle-multiplexed optical diffraction tomography for high throughput 3D imaging of cells", Proc. SPIE 11294, Emerging Digital Micromirror Device Based Systems and Applications XII, 1129402 (28 February 2020); https://doi.org/10.1117/12.2547017

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