Orientation of swimming cells with annular beam optical tweezers (Conference Presentation)

Declan Armstrong; Isaac C. Lenton; Halina Rubinsztein-Dunlop; Timo A. Nieminen; Alexander B. Stilgoe; Anatoli V. Kashchuk

doi:10.1117/12.2554512

9 March 2020 Orientation of swimming cells with annular beam optical tweezers (Conference Presentation)

Declan Armstrong, Isaac C. Lenton, Halina Rubinsztein-Dunlop, Timo A. Nieminen, Alexander B. Stilgoe, Anatoli V. Kashchuk

Author Affiliations +

Proceedings Volume 11297, Complex Light and Optical Forces XIV; 112970A (2020) https://doi.org/10.1117/12.2554512
Event: SPIE OPTO, 2020, San Francisco, California, United States

Abstract

We use dynamically controlled annular beam optical tweezers to orientate non-spherical swimming E.coli. Typically elliptically shaped particles in a Gaussian optical trap will align themselves with the direction of beam propagation. This orientation makes determining the dynamics of swimming particles difficult, as most optical tweezers systems are only able to capture information in the focal plane, but not the axial direction. We use simulations and experimental measurements to show that we are able to orientate motile E.coli from a vertical to horizontal position with a spatial light modulator in as little as one intermediate step without reduction in trap stiffness.

Conference Presentation

Citation Download Citation

Declan Armstrong, Isaac C. Lenton, Halina Rubinsztein-Dunlop, Timo A. Nieminen, Alexander B. Stilgoe, and Anatoli V. Kashchuk "Orientation of swimming cells with annular beam optical tweezers (Conference Presentation)", Proc. SPIE 11297, Complex Light and Optical Forces XIV, 112970A (9 March 2020); https://doi.org/10.1117/12.2554512

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