Dual-color quantum dot structured illumination microscopy using single LED

Hui Zeng; Huaidong Yang

doi:10.1117/12.2545456

18 December 2019 Dual-color quantum dot structured illumination microscopy using single LED

Hui Zeng, Huaidong Yang

Proceedings Volume 11337, AOPC 2019: Optical Spectroscopy and Imaging; 113370T (2019) https://doi.org/10.1117/12.2545456
Event: Applied Optics and Photonics China (AOPC2019), 2019, Beijing, China

Abstract

Structured illumination microscopy (SIM) is a kind of wide-field super-resolution (SR) imaging technique which can double the resolution than convenient microscope. Because it doesn’t need any special fluorescent labels and high illumination intensity, it’s an ideal technique for high frame multicolor subcellular imaging in large field of view. Nevertheless, most of the previous multicolor SIM schemes use multiple excitation light to excite different fluorescence labels sequentially, not only making the system complex, but also limiting the ability to observe dynamic interaction between subcellular structures. Quantum dot (QD) is a kind of novel fluorescence label which has a wide absorption spectrum. Utilizing this special property of QD, we present a simple solution of multicolor SIM. The solution uses only a single LED as excitation light source and keep the ability to simultaneous image different colors. To demonstrate the capabilities of our system, we imaged the Blue-ray disc labeled by dual-color QDs.

Citation Download Citation

Hui Zeng and Huaidong Yang "Dual-color quantum dot structured illumination microscopy using single LED", Proc. SPIE 11337, AOPC 2019: Optical Spectroscopy and Imaging, 113370T (18 December 2019); https://doi.org/10.1117/12.2545456

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