Multiparametric polarization histology in the detection of traumatic changes in the optical anisotropy of biological tissues

A. Litvinenko; M. Garazdyuk; V. Bachinsky; O. Vanchulyak; A. Ushenko; Yu. Ushenko; A. Dubolazov; P. Gorodensky; O. Yatsko; Bin Lin; Zhebo Chen

doi:10.1117/12.2568408

21 August 2020 Multiparametric polarization histology in the detection of traumatic changes in the optical anisotropy of biological tissues

A. Litvinenko, M. Garazdyuk, V. Bachinsky, O. Vanchulyak, A. Ushenko, Yu. Ushenko, A. Dubolazov, P. Gorodensky, O. Yatsko, Bin Lin, Zhebo Chen

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Proceedings Volume 11510, Applications of Digital Image Processing XLIII; 115102O (2020) https://doi.org/10.1117/12.2568408
Event: SPIE Optical Engineering + Applications, 2020, Online Only

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Abstract

For a high-precision objective histological determination of the prescription of damage to internal organs over a long period of time, a systematic approach was used based on digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods for studying temporary changes in the molecular and polycrystalline structure of brain, liver and kidney samples in the post-mortal period. It was revealed that a linear change in the magnitude of statistical moments of the 1st - 4th orders characterizing the distribution of data of digital azimuthally invariant polarizing, Mueller-matrix and tomographic methods is interconnected with the duration of damage to internal organs. On this basis, a new algorithm for digital histological determination of the prescription of the occurrence of damage is proposed. To determine the extent of damage, the method of azimuthally invariant polarization microscopy with different magnification of the image of histological sections of tissues of internal organs was used, which provided diagnostic relationships between changes in the magnitude of statistical moments of the 1st to 4th orders, which characterize the azimuth and elliptic polarization maps of digital microscopic images and time intervals of damage duration.

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A. Litvinenko, M. Garazdyuk, V. Bachinsky, O. Vanchulyak, A. Ushenko, Yu. Ushenko, A. Dubolazov, P. Gorodensky, O. Yatsko, Bin Lin, and Zhebo Chen "Multiparametric polarization histology in the detection of traumatic changes in the optical anisotropy of biological tissues", Proc. SPIE 11510, Applications of Digital Image Processing XLIII, 115102O (21 August 2020); https://doi.org/10.1117/12.2568408

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