Quantitative evaluation of TIE-based fluorescence imaging

Xiaotong Zhu; Sudheesh K. Rajput; Manoj Kumar; Xiangyu Quan; Yasuhiro Awatsuji; Osamu Matoba

doi:10.1117/12.2573913

10 October 2020 Quantitative evaluation of TIE-based fluorescence imaging

Xiaotong Zhu, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, Osamu Matoba

Author Affiliations +

Proceedings Volume 11548, Optical Design and Testing X; 1154804 (2020) https://doi.org/10.1117/12.2573913
Event: SPIE/COS Photonics Asia, 2020, Online Only

Abstract

In this work, we evaluate quantitatively the three-dimensional fluorescence imaging based transport of intensity equation (TIE). In the TIE-based fluorescence imaging, phase information from the recorded defocus fluorescence images is retrieved by solving the phase retrieval algorithm. From the retrieved phase data and their corresponding fluorescence image, the complex function can reconstructed. Focus images at different distances can be obtained after applying numerical inverse Fresnel propagation of complex function. For the quantitative evaluation of retrieved focus images, we calculate cross correlation coefficients to check intensity conservation.

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Xiaotong Zhu, Sudheesh K. Rajput, Manoj Kumar, Xiangyu Quan, Yasuhiro Awatsuji, and Osamu Matoba "Quantitative evaluation of TIE-based fluorescence imaging", Proc. SPIE 11548, Optical Design and Testing X, 1154804 (10 October 2020); https://doi.org/10.1117/12.2573913

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