An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays

X. Michalet

doi:10.1117/12.2577747

5 March 2021 An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays

X. Michalet

Author Affiliations +

Proceedings Volume 11648, Multiphoton Microscopy in the Biomedical Sciences XXI; 116480E (2021) https://doi.org/10.1117/12.2577747
Event: SPIE BiOS, 2021, Online Only

Abstract

Time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of IRF offset, decay truncation and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented.

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X. Michalet "An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays", Proc. SPIE 11648, Multiphoton Microscopy in the Biomedical Sciences XXI, 116480E (5 March 2021); https://doi.org/10.1117/12.2577747

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