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We propose a multimodal quantitative imaging technique by integrating off-axis interferometric phase microscopy (IPM) and fluorescence microscopy, for measuring morphological changes of cells induced by hyper-osmotic pressure.
Based on the collected data, we calculated the temporal dependence of various physiological parameters during osmotic pressure induced by the cell surrounding-medium change. This technique is expected to be a useful for observing the dynamics of live cells, while obtaining both quantitative imaging capabilities and molecular specificity of specific cellular organelles.
Almog Taieb,Itay Barnea, andNatan Tzvi Shaked
"Hybridization of quantitative phase microscopy and fluorescent microscopy in measurements of cells under osmotic stress", Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550U (5 March 2021); https://doi.org/10.1117/12.2577827
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Almog Taieb, Itay Barnea, Natan Tzvi Shaked, "Hybridization of quantitative phase microscopy and fluorescent microscopy in measurements of cells under osmotic stress," Proc. SPIE 11655, Label-free Biomedical Imaging and Sensing (LBIS) 2021, 116550U (5 March 2021); https://doi.org/10.1117/12.2577827