Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes

Paula Martínez-Pérez; Salvador Ponce-Alcantara; Nieves Murillo; Ana Pérez-Márquez; Jon Maudes; Inés Peraile; Laura González-López; Matilde Gil-García; Paloma Lorenzo-Lozano; Jaime Garcia-Ruperez

doi:10.1117/12.2576527

5 March 2021 Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes

Paula Martínez-Pérez, Salvador Ponce-Alcantara, Nieves Murillo, Ana Pérez-Márquez, Jon Maudes, Inés Peraile, Laura González-López, Matilde Gil-García, Paloma Lorenzo-Lozano, Jaime Garcia-Ruperez

Author Affiliations +

Proceedings Volume 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII; 116580E (2021) https://doi.org/10.1117/12.2576527
Event: SPIE BiOS, 2021, Online Only

Abstract

In the development of label-free and real-time optical biosensors, a straightforward and low-cost fabrication of the transducer is important, because it reduces costs and the complexity of the process. One possibility for such easy fabrication is electrospinning. It is a versatile and well-developed technique that allows the manufacturing of layers of nanofibers at a low cost with a Fabry-Pérot cavity-based optical response when illuminated with visible light. In this research, the suitability of such layers for the development of label-free and real-time optical biosensors is studied. For such aim, the nanofibers were biofunctionalized in flow with antibodies against bovine serum albumin through an intermediate layer of protein A/G. Then, BSA was flowed at a concentration of 10 µg/ml as the target analyte. As a result, biofunctionalization and biodetection processes were optically monitored in real-time successfully, demonstrating the suitability of such a simple-to-fabricate transducer for the development of label-free and real-time optical biosensors. Furthermore, in comparison with other optical transducers that require complex nanofabrication techniques, electrospun nanofibers can be deposited over vast areas to create several transducers in a single batch and at a low cost.

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Paula Martínez-Pérez, Salvador Ponce-Alcantara, Nieves Murillo, Ana Pérez-Márquez, Jon Maudes, Inés Peraile, Laura González-López, Matilde Gil-García, Paloma Lorenzo-Lozano, and Jaime Garcia-Ruperez "Low-cost electrospun nanofibers for label-free and real-time optical biodetection purposes", Proc. SPIE 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII, 116580E (5 March 2021); https://doi.org/10.1117/12.2576527

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