Airy light sheet microscope for in-vivo imaging of C. elegans

Hung-Chuan Hsu; Sunil Vyas; Kuang-Yuh Huang; Hsien-Shun Liao; Yuan Luo

doi:10.1117/12.2615973

27 October 2021 Airy light sheet microscope for in-vivo imaging of C. elegans

Hung-Chuan Hsu, Sunil Vyas, Kuang-Yuh Huang, Hsien-Shun Liao, Yuan Luo

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 119251O (2021) https://doi.org/10.1117/12.2615973
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

The feature of rapid divergence of conventional Gaussian beam restricts the field of view in light sheet microscopy. Comparison with Gaussian beam, the Airy beams provides many advantages in light sheet microscopy such as larger field of view (FOV), longer penetration path, and self-reconstruction. C. elegans is a model organism for developmental biological studies. Here, we design Airy light sheet microscope to perform in-vivo imaging of C. elegans. A spatial light modulator is used to generate 1D Airy beam for illumination purpose. Imaging performance is experimentally evaluated for our system. Present results may find important applications for studying internal structures in-vivo imaging of C. elegans.

Citation Download Citation

Hung-Chuan Hsu, Sunil Vyas, Kuang-Yuh Huang, Hsien-Shun Liao, and Yuan Luo "Airy light sheet microscope for in-vivo imaging of C. elegans", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 119251O (27 October 2021); https://doi.org/10.1117/12.2615973

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