Combined optical coherence tomography and light sheet fluorescence microscopy for embryonic imaging

Md Mobarak Karim; Ruijiao Sun; Behzad Khajavi; Manmohan Singh; Harshdeep S. Chawla; Yogeshwari S. Ambekar; Alexander W. Schill; David Mayerich; Mary E. Dickinson; Kirill V. Larin

doi:10.1117/12.2610091

2 March 2022 Combined optical coherence tomography and light sheet fluorescence microscopy for embryonic imaging

Md Mobarak Karim, Ruijiao Sun, Behzad Khajavi, Manmohan Singh, Harshdeep S. Chawla, Yogeshwari S. Ambekar, Alexander W. Schill, David Mayerich, Mary E. Dickinson, Kirill V. Larin

Author Affiliations +

Proceedings Volume 11952, Multimodal Biomedical Imaging XVII; 1195202 (2022) https://doi.org/10.1117/12.2610091
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Optical coherence tomography (OCT) and light sheet fluorescence microscopy (LSFM) are well-established imaging techniques preferred in developmental biology, e.g., embryonic imaging. However, each technique has its own drawbacks, such as resolution and molecular specificity with OCT and field-of-view (FOV) and speed with LSFM. To overcome these limitations for small animal embryo imaging, we have developed a co-aligned multimodal imaging system combining OCT and LSFM. The OCT probe and LSFM excitation beams were combined and scanned with a galvanometer-mounted mirror through the same objective lens. The light sheet thickness was ~13 μm. The LSFM collection arm consisted of a 0.8 numerical aperture water immersion objective, tube lens, and CCD camera, resulting in a transverse resolution of ~2.1 μm. The OCT system was based on a 100 kHz swept-source laser with a central wavelength of 1050 nm and had a lateral resolution of ~15 µm and an axial resolution of ~7 μm. Images of fluorescent microbeads and a fluorescent-tagged mouse embryo at gestational day 9.5 showed the capabilities of the multimodal imaging system. Since the OCT system and LSFM system were co-aligned, image registration was straightforward and enabled high-throughput multimodal imaging without the need for complex registration techniques.

Citation Download Citation

Md Mobarak Karim, Ruijiao Sun, Behzad Khajavi, Manmohan Singh, Harshdeep S. Chawla, Yogeshwari S. Ambekar, Alexander W. Schill, David Mayerich, Mary E. Dickinson, and Kirill V. Larin "Combined optical coherence tomography and light sheet fluorescence microscopy for embryonic imaging", Proc. SPIE 11952, Multimodal Biomedical Imaging XVII, 1195202 (2 March 2022); https://doi.org/10.1117/12.2610091

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available