Biological pigments as fluorescent labels for cytometry

Diether J. Recktenwald; Barbara Prezelin; Chia H. Chen; John Kimura

doi:10.1117/12.17823

1 July 1990 Biological pigments as fluorescent labels for cytometry

Diether J. Recktenwald, Barbara Prezelin, Chia H. Chen, John Kimura

Proceedings Volume 1206, New Technologies in Cytometry and Molecular Biology; (1990) https://doi.org/10.1117/12.17823
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

The use of protein-based biological pigments in combination with low molecular weight organic dye molecules allows the simultaneous detection of up to six independent components of a cell surface by antibody dependent fluorescence (immunofluorescence) excited with a single 488nm laser line. This paper describes the state of the art in multi-color immunofluorescence with 488nm Ar-ion laser excitation. It provides the data to design systems for specialized applications.

Citation Download Citation

Diether J. Recktenwald, Barbara Prezelin, Chia H. Chen, and John Kimura "Biological pigments as fluorescent labels for cytometry", Proc. SPIE 1206, New Technologies in Cytometry and Molecular Biology, (1 July 1990); https://doi.org/10.1117/12.17823

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